
Hyaluronic Acid LT Assay
Product information
Hyaluronic Acid LT Assay is an in vitro diagnostic test (IVD) for the quantitative determination of hyaluronic acid based on the latex agglutination method. This turbidimetric method can be applied to general clinical chemistry analyzers.
Hyaluronic acid (HA) is an unbranched glycosaminoglycan, a single chain of polymers of disaccharide units containing N-acetylhexosamine and hexose which is widely distributed in connective tissue and is produced mainly in mesenchymal cells. HA has several functions, e.g. lubrication in joints, prevention from bacterial invasion and internal body hydration.
HA is the best marker to date for serially assessing liver cirrhosis. Serum concentration of HA is consistent with the stage of fibrosis and thus, is useful for non-invasive hepatic fibrosis assessment. Therefore, it allows a liver fibrosis prediction.
A sample is mixed with a hyaluronic acid binding protein (HABP) and hyaluronic acid (HA) in the sample combines specifically with HABP. In order to make an insoluble aggregate, latex particles coated by anti-HABP antibody are added and the latex binds to above complex. As a result, the insoluble aggregate increases turbidity in the solution. The degree of turbidity of solution can be measured optically and is proportional to the concentration of hyaluronic acid in the sample.
Reagent 1, Reagent 2, calibrator and control are required for this assay.
All reagents, calibrators and controls are CE-certified according to Directive 98/79/EC on in vitro diagnostic medical devices.
Key Features
- Fast determination of hyaluronic acid in serum samples (10 min)
- High precision on clinical chemistry analyzers
- Key marker for fibrotic stage of chronic liver diseases
Storage
Store at 2-10° C. Do not freeze.
Literature
For more information and the latest publications please visit the Mediacenter.
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- Calibrator Set Package Insert DE
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