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Beta-Glucan Test

Beta-Glucan Test

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The Toxinometer MT-6500 based β-Glucan Test is an  in vitro  diagnostic test for the... more

Product information

The Toxinometer MT-6500 based β-Glucan Test is an in vitro diagnostic test for the quantitative determination of (1→3)-β-D-glucan in serum or plasma. The assay is based on LAL (Limulus Amebocyte lysate) cascade reactions measured by a kinetic turbidimetric method. 

Video: Product Introduction 

Test principle

Endotoxin in a sample is inactivated by heating the sample at 70°C for 10 minutes with the pretreatment solution, which contains non-ionic detergent and polymyxin B. This pretreatment also deactivates inhibitory protein substances in the sample. When the pretreated sample is mixed with the LAL reagent, (1→3)-β-D-glucan in the sample activates Factor G which initiates the cascade reactions shown in Fig. 1. The turbidity change caused by the gelation reaction is detected as transmittance change. The time taken for the transmittance to reach the threshold value is measured.  This interval is defined as gelation time (Tg, in Fig. 2) and correlates with (1→3)-β-D-glucan concentration. This concentration is automatically calculated from a standard curve, which is available for each lot of LAL reagent.

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Fig. 1 (left): Cascade reaction of LAL
Fig. 2 (right): Principle of kinetic turbidimetric method
 
 
 
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Test procedure

1. Pretreatment
0.1 mL of plasma or serum are added to 0.9 mL of pretreatment reagent. After mixing, the sample is heated at 70°C for 10 minutes and cooled on ice subsequently.

beta-glucan_test_pretreatment_3_en-2
Fig. 3: Pretreatment process

 

2. Measurement
0.2 mL of the pretreated sample is transferred into the LAL reagent vial. After mixing, the sample is inserted into the Toxinometer. The measurement starts immediately and takes 90 minutes.

beta-glucan_test_measurement_en
Fig. 4: Measurement process
 

Key Features

  • Single test reagent
  • Calibration by QR code scan
  • Quality control available
  • Simple procedure thanks to ready-to-use reagents and intuitive software
  • Quantitative β-glucan measurement by the kinetic turbidimetric method

Storage

Store at 2-10° C

Literature

For more information and the latest publications please visit the Mediacenter.

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