
Beta-Glucan Test
Product information
The β-Glucan Test is an in vitro diagnostic test for the quantitative determination of (1→3)-β-D-glucan in serum or plasma. The assay is based on LAL (Limulus Amebocyte lysate) cascade reactions measured by a kinetic turbidimetric method.
Test principle
Endotoxin in a sample is inactivated by heating the sample at 70°C for 10 minutes with the pretreatment solution, which contains non-ionic detergent and polymyxin B. This pretreatment also deactivates inhibitory protein substances in the sample. When the pretreated sample is mixed with the LAL reagent, (1→3)-β-D-glucan in the sample activates Factor G which initiates the cascade reactions shown in Fig. 1. The turbidity change caused by the gelation reaction is detected as transmittance change. The time taken for the transmittance to reach the threshold value is measured. This interval is defined as gelation time (Tg, in Fig. 2) and correlates with (1→3)-β-D-glucan concentration. This concentration is automatically calculated from a standard curve, which is available for each lot of LAL reagent.
Fig. 2 (right): Principle of kinetic turbidimetric method
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Test procedure
1. Pretreatment
0.1 mL of plasma or serum are added to 0.9 mL of pretreatment reagent. After mixing, the sample is heated at 70°C for 10 minutes and cooled on ice subsequently.

Fig. 3: Pretreatment process
2. Measurement
0.2 mL of the pretreated sample is transferred into the LAL reagent vial. After mixing, the sample is inserted into the LIMUSAVE MT-7500 or Toxinometer MT-6500. The measurement starts immediately and takes 90 minutes.

Fig. 4: Measurement process
Key Features
- Single test reagent
- Calibration by QR code scan
- Quality control available
- Simple procedure thanks to ready-to-use reagents and intuitive software
- Quantitative β-glucan measurement by the kinetic turbidimetric method
Storage
Store at 2-10° C
Literature
For more information and the latest publications please visit the Mediacenter.
Downloads / Technical information
Flyer & brochures
Package insert
- Test Package Insert CZ
- Test Package Insert DE
- Test Package Insert EN
- Test Package Insert ES
- Test Package Insert FR
- Test Package Insert HU
- Test Package Insert IT
- Test Package Insert SR
- Control Package Insert CZ
- Control Package Insert DE
- Control Package Insert EN
- Control Package Insert ES
- Control Package Insert FR
- Control Package Insert HU
- Control Package Insert IT
- Control Package Insert SR
- Sample Diluent Package Insert CZ
- Sample Diluent Package Insert DE
- Sample Diluent Package Insert EN
- Sample Diluent Package Insert ES
- Sample Diluent Package Insert FR
- Sample Diluent Package Insert HU
- Sample Diluent Package Insert IT
- Sample Diluent Package Insert SR
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