β-Glucan Test

Description

The Toxinometer MT-6500 based ß-Glucan Test is an in vitro diagnostic test for the quantitative determination of (1→3)-ß-D-glucan in serum or plasma. The assay is based on LAL (Limulus Amebocyte lysate) cascade reactions measured by a kinetic turbidimetric method. 

 

Test principle

Endotoxin in a sample is inactivated by heating the sample at 70°C for 10 minutes with the pretreatment solution, which contains non-ionic detergent and polymyxin B. This pretreatment also deactivates inhibitory protein substances in the sample. When the pretreated sample is mixed with the LAL reagent, (1→3)-ß-D-glucan in the sample activates Factor G which initiates the cascade reactions shown in Fig. 1. The turbidity change caused by the gelation reaction is detected as transmittance change. The time taken for the transmittance to reach the threshold value is measured.  This interval is defined as gelation time (Tg, in Fig. 2) and correlates with (1→3)-ß-D-glucan concentration. This concentration is automatically calculated from a standard curve, which is available for each lot of LAL reagent. 

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              Fig. 1: Cascade reaction of LAL                 Fig. 2: Principle of kinetic turbidimetric method

 

Test procedure

1. Pretreatment
0.1 mL of plasma or serum are added to 0.9 mL of pretreatment reagent. After mixing, the sample is heated at 70°C for 10 minutes and cooled on ice subsequently.

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Fig. 3: Pretreatment process

 

2. Measurement
0.2 mL of the pretreated sample is transferred into the LAL reagent vial. After mixing, the sample is inserted into the Toxinometer. The measurement starts immediately and takes 90 minutes.

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Fig. 4: Measurement process

Key Features

  • Monotest reagent
  • Calibration by QR code scan
  • Quality control available
  • Ready-to-use reagents
  • Quantitative ß-glucan measurement by kinetic turbidimetric LAL method
  • Measurement range: 6 - 600 pg/mL
  • Cut-off value: 11 pg/mL
  • High precision and no significant interference observed due to bilirubin, hemolysis or antifungal drugs

Storage

Store at 2-10° C.

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Test Components