NEFA-HR(2) Assay


NEFA-HR(2) Assay is an in vitro diagnostic (IVD) test for the quantitative determination of non-esterified fatty acids (NEFA) in serum.

NEFA binding to albumin in serum is used as an important energy source of peripheral tissues. The amount at NEFA in serum depends on a balance between intake in liver and peripheral tissues as well as on the release from adipose tissues.  It decreases by physical exercise and increases by starvation, cold, fear or smoking. An increase or decrease of NEFA is also observed in diabetes, hepatic diseases or endocrine diseases. One of the major applications of this test is the diagnostics of the metabolic syndrome.

NEFA-HR(2) Assay is based on the enzymatic method using 3-Methyl-N-Ethyl-N-(β-Hydroxyethyl)-Aniline (MEHA) as a violet color agent.

Reagent 1, Reagent 1a, Reagent 2 and Reagent 2a as well a standard are required for this assay.

All reagents and calibrators are CE-certified according to Directive 98/79/EC on in vitro diagnostic medical devices.

Key Features

  • Reliable results without interference from ascorbic acid and bilirubin
  • High linearity
  • Accurate, precise, simple and fast
  • Applicable to all common clinical chemistry analyzers and manual methods
  • Also applicable in veterinary samples


Recent studies have been performed in the veterinary field employing NEFA-HR(2) Assay on mice, sows and rabbits as well as dairy cows and beef cows.


Store at 2-10° C.


Ready-to-use Test Components
OEM test components for this assay are available on request.