Autokit CH50 Assay

Description

Autokit CH50 Assay is an in vitro diagnostic (IVD) test for the quantitative determination of total complement activity (CH50) in human serum.

The complement system is a part of the immune system that enhances/complements the ability of antibodies and phagocytic cells to clear pathogens from an organism.

Complement activity in human sera can provide important information in the diagnosis of many diseases. Clinically, the measurement of complement activity is a direct indicator of abnormalities of the complement system and differs from immunoreactive components of the system. Complement activity has been correlated with the active stage of systemic lupuserythematosus, rheumatoid arthritis, cryoglobulinemia-vasculitis, some forms of nephritis and inherited deficiencies of the complement system.

Autokit CH50 Assay is an automated liposome-based assay (LIA) for total complement using a homogeneous population of small-size liposomes (200 nm).

Reagent 1, Reagent 2 and Reagent 2a as well as a calibrator and a complement control are required for this assay.

All reagents and calibrators are CE-certified according to Directive 98/79/EC on in vitro diagnostic medical devices.

Key Features

  • Liposome immunoassay, stable and homogeneous
  • Applicable to automated analyzers
  • Precise and accurate
  • Extended calibration stability
  • Good correlation with Mayer’s hemolytic method

References

Wako Chemicals GmbH regularly participates in External Quality Assurance (EQA) tests. Useful information regarding the measurement of CH50 can be found here. Please click under “EQA area” on Clinical Chemistry and select under “Group” 246: Complement Analysis 02 (Functional analysis of the pathways). The LIA method has the code m0332.

Autokit CH50 Assay is also applied by the Ulm University Hospital in Germany. For more information (in German language only), please click here.

Storage

Store reagents at 2-10° C. Do not freeze Reagent 1.
Store CH50 Calibrator and Complement Control below -10° C.

Safety information

Health Hazard

Downloads

Ready-to-use Test Components
OEM test components for this assay are available on request.